Description：Mouse monoclonal antibody to SMAD3Immunogen：Recombinant protein corresponding to human SMAD3.Purification：Affinity chromatographyClonality：MonoclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/1000 - 1/2000), IH (1/100 - 1/200)Gene Symbol：SMAD3Alternative Names：MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3 Entrez Gene (Human)： 4088; SwissProt (Human)： P84022; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of SMAD3 expression in 293T (A), Jurkat (B), mouse heart (C), rat heart (D) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 52 kD)
• 
  Immunohistochemical analysis of SMAD3 staining in human liver,human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.