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CPA9200
  • Western blot analysis of NF-kappaB p65 expression in Hela (A), 293T (B), HepG (C) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 65 kD)
  • Immunohistochemical analysis of NF-kappaB p65 staining in mouse hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunoprecipitation of NF-kappaB p65 from 0.5mg Hela whole cell extract lysate, using Anti-NF-kappaB p65 Antibody.
Product Name:Anti-NF-kappaB p65 Antibody
Cat No:CPA9200
Source:Mouse
Reactivity:H, M, R
Applications:WB, IH, IP
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Mouse monoclonal antibody to NF-kappaB p65
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence of human NF-kappaB p65. The exact sequence is proprietary.
Purification:Affinity chromatography
Clonality:Monoclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/1000 - 1/3000), IH (1/100 - 1/200), IP (1/50 - 1/200)
Gene Symbol:RELA
Alternative Names:NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Entrez Gene (Human): 5970;
Entrez Gene (Mouse): 19697;
SwissProt (Human): Q04206;
SwissProt (Mouse): Q04207;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of NF-kappaB p65 expression in Hela (A), 293T (B), HepG (C) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 65 kD)
  • Immunohistochemical analysis of NF-kappaB p65 staining in mouse hippocampus formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunoprecipitation of NF-kappaB p65 from 0.5mg Hela whole cell extract lysate, using Anti-NF-kappaB p65 Antibody.
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