Description:Mouse monoclonal antibody to GAP43Immunogen:Recombinant protein corresponding to human GAP43.Purification:Affinity chromatographyClonality:MonoclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/1000 - 1/2000), IH (1/200 - 1/500)Gene Symbol:GAP43Alternative Names:Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46
Entrez Gene (Human):
2596;
SwissProt (Human):
P17677;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GAP43 expression in Hela (A), 293T (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 43 kD)
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Immunohistochemical analysis of GAP43 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.