Description：Mouse monoclonal antibody to FumaraseImmunogen：KLH-conjugated synthetic peptide encompassing a sequence of human Fumarase. The exact sequence is proprietary.Purification：Affinity chromatographyClonality：MonoclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200)Gene Symbol：FHAlternative Names：Fumarate hydratase mitochondrial; Fumarase Entrez Gene (Human)： 2271; Entrez Gene (Mouse)： 14194; SwissProt (Human)： P07954; SwissProt (Mouse)： P97807; SwissProt (Rat)： P14408; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Fumarase expression in 293T (A), HepG2 (B), Hela (C), mouse brain (D), rat brain (E) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 48 kD)
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  Immunofluorescent analysis of Fumarase staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).