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CPA9157
  • Western blot analysis of ERCC1 expression in Hela (A), HepG2 (B), 293T (C), Jurkat (D) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 36 kD)
  • Immunohistochemical analysis of ERCC1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-ERCC1 Antibody
Cat No:CPA9157
Source:Mouse
Reactivity:H
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Mouse monoclonal antibody to ERCC1
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence of human ERCC1. The exact sequence is proprietary.
Purification:Affinity chromatography
Clonality:Monoclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/1000 - 1/2000), IH (1/100 - 1/200)
Gene Symbol:ERCC1
Alternative Names:DNA excision repair protein ERCC-1
Entrez Gene (Human): 2067;
SwissProt (Human): P07992;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of ERCC1 expression in Hela (A), HepG2 (B), 293T (C), Jurkat (D) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 36 kD)
  • Immunohistochemical analysis of ERCC1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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