Description：Mouse monoclonal antibody to eNOSImmunogen：Recombinant protein corresponding to human eNOS.Purification：Affinity chromatographyClonality：MonoclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IH (1/200 - 1/500)Gene Symbol：NOS3Alternative Names：Nitric oxide synthase endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII Entrez Gene (Human)： 4846; Entrez Gene (Mouse)： 18127; Entrez Gene (Rat)： 24600; SwissProt (Human)： P29474; SwissProt (Mouse)： P70313; SwissProt (Rat)： Q62600; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of eNOS expression in rat heart (A) whole cell lysates. (Predicted band size: 133; 69 kD; Observed band size: 133 kD)
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  Immunohistochemical analysis of eNOS staining in mouse colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.