Description：Mouse monoclonal antibody to EGFRImmunogen：KLH-conjugated synthetic peptide encompassing a sequence of human EGFR. The exact sequence is proprietary.Purification：Affinity chromatographyClonality：MonoclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200), IP (1/100 - 1/200)Gene Symbol：EGFRAlternative Names：ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 Entrez Gene (Human)： 1956; SwissProt (Human)： P00533; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EGFR expression in A431 (A), Hela (B) whole cell lysates. (Predicted band size: 134 kD; Observed band size: 175 kD)
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  Immunofluorescent analysis of EGFR staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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  Immunoprecipitation of EGFR from 0.5mg Hela whole cell extract lysate, using Anti-EGFR Antibody.