Description:Mouse monoclonal antibody to Cytochrome cImmunogen:Recombinant protein corresponding to human Cytochrome c.Purification:Affinity chromatographyClonality:MonoclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/1000 - 1/3000), IH (1/200 - 1/500)Gene Symbol:CYCSAlternative Names:CYC; Cytochrome c
Entrez Gene (Human):
54205;
SwissProt (Human):
P99999;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Cytochrome c expression in Hela (A), 293T (B), NIH3T3 (C), mouse liver (D), rat liver (E), rat kidney (F) whole cell lysates. (Predicted band size: 11 kD; Observed band size: 14 kD)
-
Immunohistochemical analysis of Cytochrome c staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.