Datasheet
MSDSDescription：Mouse monoclonal antibody to COX4-1Immunogen：Recombinant protein corresponding to human COX4-1.Purification：Affinity chromatographyClonality：MonoclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/1000 - 1/3000), IF/IC (1/100 - 1/200)Gene Symbol：COX4I1Alternative Names：COX4; Cytochrome c oxidase subunit 4 isoform 1 mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1
Entrez Gene (Human)：
1327;
Entrez Gene (Mouse)：
12857;
Entrez Gene (Rat)：
29445;
SwissProt (Human)：
P13073;
SwissProt (Mouse)：
P19783;
SwissProt (Rat)：
P10888;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of COX4-1 expression in Hela (A), HepG2 (B), 293T (C), mouse heart (D), mouse muscle (E), rat heart (F) whole cell lysates. (Predicted band size: 19 kD; Observed band size: 15 kD) -
Immunofluorescent analysis of COX4-1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).