Description:Mouse monoclonal antibody to Beta-cateninImmunogen:KLH-conjugated synthetic peptide encompassing a sequence of human Beta-catenin. The exact sequence is proprietary.Purification:Affinity chromatographyClonality:MonoclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/1000 - 1/2000), IH (1/100 - 1/200)Gene Symbol:CTNNB1Alternative Names:CTNNB; Catenin beta-1; Beta-catenin
Entrez Gene (Human):
1499;
Entrez Gene (Mouse):
12387;
Entrez Gene (Rat):
84353;
SwissProt (Human):
P35222;
SwissProt (Mouse):
Q02248;
SwissProt (Rat):
Q9WU82;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Beta-catenin expression in Hela (A), 293T (B), MCF7 (C), mouse brain (D), rat liver (E) whole cell lysates. (Predicted band size: 85 kD; Observed band size: 92 kD)
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Immunohistochemical analysis of Beta-catenin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.