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CPA9114
  • Western blot analysis of Beta1-tubulin expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 52 kD)
  • Immunohistochemical analysis of Beta1-tubulin staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-Beta1-tubulin Antibody
Cat No:CPA9114
Source:Mouse
Reactivity:H, M, R
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
130
100 μl
80
30 μl
40
50 μl
55
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Description:Mouse monoclonal antibody to Beta1-tubulin
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence of human Beta1-tubulin. The exact sequence is proprietary.
Purification:Affinity chromatography
Clonality:Monoclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/5000 - 1/10000), IH (1/200 - 1/500)
Gene Symbol:TUBB1
Alternative Names:Tubulin beta-1 chain
Entrez Gene (Human): 81027;
Entrez Gene (Mouse): 545486;
SwissProt (Human): Q9H4B7;
SwissProt (Mouse): A2AQ07;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Beta1-tubulin expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 52 kD)
  • Immunohistochemical analysis of Beta1-tubulin staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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