Description:Mouse monoclonal antibody to AMACRImmunogen:KLH-conjugated synthetic peptide encompassing a sequence of human AMACR. The exact sequence is proprietary.Purification:Affinity chromatographyClonality:MonoclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/1000 - 1/2000), IH (1/100 - 1/200)Gene Symbol:AMACRAlternative Names:Alpha-methylacyl-CoA racemase; 2-methylacyl-CoA racemase
Entrez Gene (Human):
23600;
Entrez Gene (Mouse):
17117;
SwissProt (Human):
Q9UHK6;
SwissProt (Mouse):
O09174;
SwissProt (Rat):
P70473;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of AMACR expression in HepG2 (A), mouse kidney (B) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 42 kD)
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Immunohistochemical analysis of AMACR staining in mouse prostate adenocancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.