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CPA7470
  • Western blot analysis of Cytokeratin 14/17 expression in HepG2 (A), A431 (B) whole cell lysates. (Predicted band size: 51; 48 kD; Observed band size: 52 kD)
  • Immunohistochemical analysis of Cytokeratin 14/17 staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-Cytokeratin 14/17 Antibody
Cat No:CPA7470
Source:Rabbit
Reactivity:H, M, R, B
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
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200 μl
350
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30 μl
110
50 μl
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Description:Rabbit polyclonal antibody to Cytokeratin 14/17
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytokeratin 14/17. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)
Gene Symbol:KRT14; KRT17
Alternative Names:KRT14; Keratin type I cytoskeletal 14; Cytokeratin-14; CK-14; Keratin-14; K14; KRT17; Keratin type I cytoskeletal 17; 39.1; Cytokeratin-17; CK-17; Keratin-17; K17
Entrez Gene (Human): 3861; 3872;
Entrez Gene (Mouse): 16664; 16667;
Entrez Gene (Rat): 287701; 287702;
SwissProt (Human): P02533; Q04695;
SwissProt (Mouse): Q61781; Q9QWL7;
SwissProt (Rat): Q6IFV1; Q6IFU8;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Cytokeratin 14/17 expression in HepG2 (A), A431 (B) whole cell lysates. (Predicted band size: 51; 48 kD; Observed band size: 52 kD)
  • Immunohistochemical analysis of Cytokeratin 14/17 staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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