Description:Rabbit polyclonal antibody to ATMImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATM. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:ATMAlternative Names:Serine-protein kinase ATM; Ataxia telangiectasia mutated; A-T mutated
Entrez Gene (Human):
472;
Entrez Gene (Mouse):
11920;
SwissProt (Human):
Q13315;
SwissProt (Mouse):
Q62388;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ATM expression in K562 (A), mouse brain (B) whole cell lysates. (Predicted band size: 350 kD; Observed band size: 350 kD)
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Immunohistochemical analysis of ATM staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.