Description:Rabbit polyclonal antibody to TRB1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TRB1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol:TAS2R14Alternative Names:Taste receptor type 2 member 14; T2R14; Taste receptor family B member 1; TRB1
Entrez Gene (Human):
50840;
SwissProt (Human):
Q9NYV8;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TRB1 expression in Hela (A), COLO205 (B), L929 (C) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 36 kD)
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Immunofluorescent analysis of TRB1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).