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CPA7272
  • Western blot analysis of VEGFA expression in Hela (A), A549 (B), mouse liver (C), rat liver (D) whole cell lysates. (Predicted band size: 27 kD; Observed band size: 17; 38; 40; 45 kD)
  • Immunohistochemical analysis of VEGFA staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-VEGFA Antibody
Cat No:CPA7272
Source:Rabbit
Reactivity:H, M, R, P
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to VEGFA
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human VEGFA. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)
Gene Symbol:VEGFA
Alternative Names:VEGF; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF
Entrez Gene (Human): 7422;
Entrez Gene (Mouse): 22339;
Entrez Gene (Rat): 83785;
SwissProt (Human): P15692;
SwissProt (Mouse): Q00731;
SwissProt (Rat): P16612;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of VEGFA expression in Hela (A), A549 (B), mouse liver (C), rat liver (D) whole cell lysates. (Predicted band size: 27 kD; Observed band size: 17; 38; 40; 45 kD)
  • Immunohistochemical analysis of VEGFA staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Breast cancer cell-derived extracellular vesicles promote human umbilical vein endothelial cells proliferation and migration by regulating the JAK2/STAT3 pathway
Journal Research
IF 11
Application WB
Reactivity Human
PMID
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