Description：Rabbit polyclonal antibody to HMGB1Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human HMGB1. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol：HMGB1Alternative Names：HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1 Entrez Gene (Human)： 3146; Entrez Gene (Mouse)： 100862258; 15289; Entrez Gene (Rat)： 25459; SwissProt (Human)： P09429; SwissProt (Mouse)： P63158; SwissProt (Rat)： P63159; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HMGB1 expression in HepG2 UV-treated (A) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)
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  Immunohistochemical analysis of HMGB1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.