Description：Rabbit polyclonal antibody to HMGB1 (Acetyl-K12)Immunogen：KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K12 of human HMGB1 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000)Gene Symbol：HMGB1Alternative Names：HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1 Entrez Gene (Human)： 3146; Entrez Gene (Mouse)： 100862258; 15289; Entrez Gene (Rat)： 25459; SwissProt (Human)： P09429; SwissProt (Mouse)： P63158; SwissProt (Rat)： P63159; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HMGB1 (Acetyl-K12) expression in DLD (A), rat liver (B) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)
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  Direct ELISA antibody dose-response curve using Anti-HMGB1 (Acetyl-K12) Antibody. Antigen (Acetyl-peptide and non-acetyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.