Description：Rabbit polyclonal antibody to AKT2 (Phospho-S474)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S474 of human AKT2 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol：AKT2Alternative Names：RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta Entrez Gene (Human)： 208; Entrez Gene (Mouse)： 11652; Entrez Gene (Rat)： 25233; SwissProt (Human)： P31751; SwissProt (Mouse)： Q60823; SwissProt (Rat)： P47197; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of AKT2 (Phospho-S474) expression in Hela (A), mouse brain (B), PC12 UV-treated (C) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 60 kD)
• 
  Immunohistochemical analysis of AKT2 (Phospho-S474) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.