Description:Rabbit polyclonal antibody to PLEKHG4Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PLEKHG4. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:PLEKHG4Alternative Names:PRTPHN1; Puratrophin-1; Pleckstrin homology domain-containing family G member 4; PH domain-containing family G member 4; Purkinje cell atrophy-associated protein 1
Entrez Gene (Human):
25894;
SwissProt (Human):
Q58EX7;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PLEKHG4 expression in SHSY5Y (A), HEK293T (B), K562 (C) whole cell lysates. (Predicted band size: 130 kD; Observed band size: 131 kD)
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Immunohistochemical analysis of PLEKHG4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.