Description：Rabbit polyclonal antibody to PERKImmunogen：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PERK. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol：EIF2AK3Alternative Names：PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK Entrez Gene (Human)： 9451; Entrez Gene (Rat)： 29702; SwissProt (Human)： Q9NZJ5; SwissProt (Mouse)： Q9Z2B5; SwissProt (Rat)： Q9Z1Z1; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PERK expression in A549 (A), MCF7 (B), 3T3 (C) whole cell lysates. (Predicted band size: 125 kD; Observed band size: 125 kD)
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  Immunohistochemical analysis of PERK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.