Description:Rabbit polyclonal antibody to BTKImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BTK. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol:BTKAlternative Names:AGMX1; ATK; BPK; Tyrosine-protein kinase BTK; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase
Entrez Gene (Human):
695;
Entrez Gene (Mouse):
12229;
SwissProt (Human):
Q06187;
SwissProt (Mouse):
P35991;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of BTK expression in Hela (A), mouse brain (B) whole cell lysates. (Predicted band size: 76 kD; Observed band size: 75 kD)
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Immunohistochemical analysis of BTK staining in human spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.