Description：Rabbit polyclonal antibody to Estrogen Receptor alpha (Phospho-S104)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S104 of human Estrogen Receptor alpha protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200)Gene Symbol：ESR1Alternative Names：ESR; NR3A1; Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1 Entrez Gene (Human)： 2099; Entrez Gene (Mouse)： 13982; Entrez Gene (Rat)： 24890; SwissProt (Human)： P03372; SwissProt (Mouse)： P19785; SwissProt (Rat)： P06211; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Estrogen Receptor alpha (Phospho-S104) expression in HEK293T (A), Hela (B), MCF7 (C) whole cell lysates. (Predicted band size: 66 kD; Observed band size: 66 kD)
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  Immunohistochemical analysis of Estrogen Receptor alpha (Phospho-S104) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.