Description：Rabbit polyclonal antibody to BRSK1Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BRSK1. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：BRSK1Alternative Names：KIAA1811; SAD1; SADB; Serine/threonine-protein kinase BRSK1; Brain-selective kinase 1; Brain-specific serine/threonine-protein kinase 1; BR serine/threonine-protein kinase 1; Serine/threonine-protein kinase SAD-B; Synapses of Amphids Defective homolog 1; SAD1 homolog; hSAD1 Entrez Gene (Human)： 84446; Entrez Gene (Mouse)： 381979; SwissProt (Human)： Q8TDC3; SwissProt (Mouse)： Q5RJI5; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of BRSK1 expression in Hela (A), CT26 (B) whole cell lysates. (Predicted band size: 85 kD; Observed band size: 87 kD)
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  Immunofluorescent analysis of BRSK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.