Description：Rabbit polyclonal antibody to PKC theta (Phospho-T538)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T538 of human PKC theta protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol：PRKCQAlternative Names：PRKCT; Protein kinase C theta type; nPKC-theta Entrez Gene (Human)： 5588; Entrez Gene (Mouse)： 18761; SwissProt (Human)： Q04759; SwissProt (Mouse)： Q02111; SwissProt (Rat)： Q9WTQ0; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PKC theta (Phospho-T538) expression in mouse muscle (A), rat muscle (B) whole cell lysates. (Predicted band size: 81 kD; Observed band size: 82 kD)
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  Immunohistochemical analysis of PKC theta (Phospho-T538) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.