Description:Rabbit polyclonal antibody to Histone H4 (MonoMethyl-R3)Immunogen:KLH-conjugated synthetic Mono-methylated peptide corresponding to residues surrounding R3 of human Histone H4 protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000)Gene Symbol:HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; HIST4H4Alternative Names:H4/A; H4FA; H4/I; H4FI; H4/G; H4FG; H4/B; H4FB; H4/J; H4FJ; H4/C; H4FC; H4/H; H4FH; H4/M; H4FM; H4/E; H4FE; H4/D; H4FD; H4/K; H4FK; H4/N; H4F2; H4FN; HIST2H4; H4/O; H4FO; Histone H4
Entrez Gene (Human):
121504;
554313;
8294;
8359;
8360;
8361;
8362;
8363;
8364;
8365;
8366;
8367;
8368;
8370;
Entrez Gene (Mouse):
100041230;
102641229;
319155;
319156;
319157;
319158;
319159;
319160;
319161;
320332;
326619;
326620;
69386;
97122;
Entrez Gene (Rat):
100360950;
100912290;
100912418;
100912564;
102548682;
102551184;
102557184;
291152;
295277;
500351;
502913;
64627;
680097;
SwissProt (Human):
P62805;
SwissProt (Mouse):
P62806;
SwissProt (Rat):
P62804;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Histone H4 (MonoMethyl-R3) expression in HEK293T (A), A549 (B), U2OS (C) whole cell lysates. (Predicted band size: 11 kD; Observed band size: 14 kD)
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Direct ELISA antibody dose-response curve using Anti-Histone H4 (MonoMethyl-R3) Antibody. Antigen (methyl-peptide and non-methyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.