Description：Rabbit polyclonal antibody to Orexin Receptor 1Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Orexin Receptor 1. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：HCRTR1Alternative Names：Orexin receptor type 1; Ox-1-R; Ox1-R; Ox1R; Hypocretin receptor type 1 Entrez Gene (Human)： 3061; Entrez Gene (Mouse)： 230777; Entrez Gene (Rat)： 25593; SwissProt (Human)： O43613; SwissProt (Mouse)： P58307; SwissProt (Rat)： P56718; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Orexin Receptor 1 expression in U87MG (A), rat brain (B), mouse brain (C) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 55 kD)
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  Immunofluorescent analysis of Orexin Receptor 1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.