Description：Rabbit polyclonal antibody to MDM2 (Phospho-S186/S188)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S186/S188 of human MDM2 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：MDM2Alternative Names：E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2 Entrez Gene (Human)： 4193; SwissProt (Human)： Q00987; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MDM2 (Phospho-S186/S188) expression in SGC7901 (A), Panc1 (B) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 100 kD)
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  Immunofluorescent analysis of MDM2 (Phospho-S186/S188) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.