Description：Rabbit polyclonal antibody to CD4 (Phospho-S433)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S433 of human CD4 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：CD4Alternative Names：T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4 Entrez Gene (Human)： 920; Entrez Gene (Mouse)： 12504; Entrez Gene (Rat)： 24932; SwissProt (Human)： P01730; SwissProt (Mouse)： P06332; SwissProt (Rat)： P05540; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD4 (Phospho-S433) expression in mouse spleen (A), rat spleen (B) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 56 kD)
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  Immunofluorescent analysis of CD4 (Phospho-S433) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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  Direct ELISA antibody dose-response curve using Anti-CD4 (Phospho-S433) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.