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CPA6352
  • Western blot analysis of Aquaporin 2 (Phospho-S256) expression in mouse kidney (A), rat kidney (B), mouse lung (C) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 37; 28 kD)
  • Immunohistochemical analysis of Aquaporin 2 (Phospho-S256) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-Aquaporin 2 (Phospho-S256) Antibody
Cat No:CPA6352
Source:Rabbit
Reactivity:H, M, R, B, D, P, S
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
420
100 μl
260
30 μl
130
50 μl
170
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Description:Rabbit polyclonal antibody to Aquaporin 2 (Phospho-S256)
Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S256 of human Aquaporin 2 protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)
Gene Symbol:AQP2
Alternative Names:Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct
Entrez Gene (Human): 359;
Entrez Gene (Mouse): 11827;
Entrez Gene (Rat): 25386;
SwissProt (Human): P41181;
SwissProt (Mouse): P56402;
SwissProt (Rat): P34080;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Aquaporin 2 (Phospho-S256) expression in mouse kidney (A), rat kidney (B), mouse lung (C) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 37; 28 kD)
  • Immunohistochemical analysis of Aquaporin 2 (Phospho-S256) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Arginine vasopressin and angiotensin II coregulate Aquaporin 2 expression in M-1 cells
Journal Biochem Biophys Res Commun
IF 2.5
Application WB, IF
Reactivity Mouse
PMID 39740402
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