Description:Rabbit polyclonal antibody to Laminin alpha 3Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Laminin alpha 3. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol:LAMA3Alternative Names:LAMNA; Laminin subunit alpha-3; Epiligrin 170 kDa subunit; E170; Epiligrin subunit alpha; Kalinin subunit alpha; Laminin-5 subunit alpha; Laminin-6 subunit alpha; Laminin-7 subunit alpha; Nicein subunit alpha
Entrez Gene (Human):
3909;
Entrez Gene (Mouse):
16774;
SwissProt (Human):
Q16787;
SwissProt (Mouse):
Q61789;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Laminin alpha 3 expression in AML12 (A), HEK293T (B), SGC7901 (C) whole cell lysates. (Predicted band size: 366 kD; Observed band size: 189 kD)
-
Immunofluorescent analysis of Laminin alpha 3 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.