Description:Rabbit polyclonal antibody to BACE1 (Acetyl-K316)Immunogen:KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K316 of human BACE1 protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:BACE1Alternative Names:BACE; KIAA1149; Beta-secretase 1; Aspartyl protease 2; ASP2; Asp 2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2
Entrez Gene (Human):
23621;
Entrez Gene (Mouse):
23821;
SwissProt (Human):
P56817;
SwissProt (Mouse):
P56818;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of BACE1 (Acetyl-K316) expression in MCF7 (A), SKOVCAR3 (B), mouse brain (C) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 55 kD)
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Immunohistochemical analysis of BACE1 (Acetyl-K316) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.