Description：Rabbit polyclonal antibody to TRHRImmunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TRHR. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol：TRHRAlternative Names：Thyrotropin-releasing hormone receptor; TRH-R; Thyroliberin receptor Entrez Gene (Human)： 7201; Entrez Gene (Mouse)： 22045; Entrez Gene (Rat)： 25570; SwissProt (Human)： P34981; SwissProt (Mouse)： P21761; SwissProt (Rat)： Q01717; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TRHR expression in U87MG (A), mouse brain (B), mouse kidney (C) whole cell lysates. (Predicted band size: 45 kD; Observed band size: 55; 45 kD)
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  Immunofluorescent analysis of TRHR staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.