Description：Rabbit polyclonal antibody to Endothelin A ReceptorImmunogen：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Endothelin A Receptor. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol：EDNRAAlternative Names：ETA; ETRA; Endothelin-1 receptor; Endothelin A receptor; ET-A; ETA-R; hET-AR Entrez Gene (Human)： 1909; Entrez Gene (Mouse)： 13617; SwissProt (Human)： P25101; SwissProt (Mouse)： Q61614; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of Endothelin A Receptor expression in AML12 (A), Hela (B), A2780 (C), HepG2 (D) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 69; 55 kD)
• 
  Immunofluorescent analysis of Endothelin A Receptor staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.