Description：Rabbit polyclonal antibody to 5-HT4Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human 5-HT4. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol：HTR4Alternative Names：5-hydroxytryptamine receptor 4; 5-HT-4; 5-HT4; Serotonin receptor 4 Entrez Gene (Human)： 3360; Entrez Gene (Mouse)： 15562; Entrez Gene (Rat)： 25324; SwissProt (Human)： Q13639; SwissProt (Mouse)： P97288; SwissProt (Rat)： Q62758; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of 5-HT4 expression in U87MG (A), rat heart (B) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 42 kD)
• 
  Immunofluorescent analysis of 5-HT4 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.