Description:Rabbit polyclonal antibody to ELOA3Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ELOA3. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol:ELOA3; ELOA3B; ELOA3DAlternative Names:TCEB3B; TCEB3L; RNA polymerase II transcription factor SIII subunit A2; Elongin-A2; EloA2; Transcription elongation factor B polypeptide 3B; TCEB3C; TCEB3L2; RNA polymerase II transcription factor SIII subunit A3; Elongin-A3; EloA3; Transcription elongation factor B polypeptide 3C
Entrez Gene (Human):
51224;
162699;
SwissProt (Human):
Q8IYF1;
Q8NG57;
Q3SY89;
A6NLF2;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of ELOA3 expression in SKOVCAR3 (A), Panc1 (B) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 59 kD)
-
Immunofluorescent analysis of ELOA3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.