Description:Rabbit polyclonal antibody to BWR1AImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BWR1A. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)Gene Symbol:SLC22A18Alternative Names:BWR1A; BWSCR1A; HET; IMPT1; ITM; ORCTL2; SLC22A1L; TSSC5; Solute carrier family 22 member 18; Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene A protein; Efflux transporter-like protein; Imprinted multi-membrane-spanning polyspecific transporter-related protein 1; Organic cation transporter-like protein 2; ORCTL-2; Solute carrier family 22 member 1-like; Tumor-suppressing STF cDNA 5 protein; Tumor-suppressing subchromosomal transferable fragment candidate gene 5 protein; p45-Beckwith-Wiedemann region 1 A; p45-BWR1A
Entrez Gene (Human):
5002;
Entrez Gene (Mouse):
18400;
Entrez Gene (Rat):
309131;
SwissProt (Human):
Q96BI1;
SwissProt (Mouse):
Q78KK3;
SwissProt (Rat):
Q6AY78;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of BWR1A expression in C6 (A), CT26 (B), Hela (C), A2780 (D) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 40; 44 kD)
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Immunofluorescent analysis of BWR1A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.