Description:Rabbit polyclonal antibody to EVI1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human EVI1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol:MECOMAlternative Names:MDS1; MDS1 and EVI1 complex locus protein MDS1; Myelodysplasia syndrome 1 protein; Myelodysplasia syndrome-associated protein 1
Entrez Gene (Human):
2122;
SwissProt (Human):
Q03112;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EVI1 expression in A549 (A), SGC7901 (B) whole cell lysates. (Predicted band size: 138 kD; Observed band size: 138 kD)
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Immunohistochemical analysis of EVI1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.