Description:Rabbit polyclonal antibody to DAP Kinase 1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DAP Kinase 1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/100)Gene Symbol:DAPK1Alternative Names:DAPK; Death-associated protein kinase 1; DAP kinase 1
Entrez Gene (Human):
1612;
Entrez Gene (Mouse):
69635;
SwissProt (Human):
P53355;
SwissProt (Mouse):
Q80YE7;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DAP Kinase 1 expression in C6 (A), CT26 (B) whole cell lysates. (Predicted band size: 160 kD; Observed band size: 160 kD)
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Immunohistochemical analysis of DAP Kinase 1 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.