Description:Rabbit polyclonal antibody to Lamin A/C (Phospho-S22)Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S22 of human Lamin A/C protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol:LMNAAlternative Names:LMN1; Prelamin-A/C
Entrez Gene (Human):
4000;
Entrez Gene (Mouse):
16905;
Entrez Gene (Rat):
60374;
SwissProt (Human):
P02545;
SwissProt (Mouse):
P48678;
SwissProt (Rat):
P48679;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Lamin A/C (Phospho-S22) expression in A375 (A) whole cell lysates. (Predicted band size: 74 kD; Observed band size: 78; 69 kD)
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Immunohistochemical analysis of Lamin A/C (Phospho-S22) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.