Description：Rabbit polyclonal antibody to p53 (Phospho-T387)Immunogen：KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T387 of human p53 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol：TP53Alternative Names：P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53 Entrez Gene (Human)： 7157; SwissProt (Human)： P04637; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of p53 (Phospho-T387) expression in EC9706 (A) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 53 kD)
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  Immunohistochemical analysis of p53 (Phospho-T387) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.