Description:Rabbit polyclonal antibody to CD166Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD166. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol:ALCAMAlternative Names:MEMD; CD166 antigen; Activated leukocyte cell adhesion molecule; CD166
Entrez Gene (Human):
214;
Entrez Gene (Mouse):
11658;
Entrez Gene (Rat):
79559;
SwissProt (Human):
Q13740;
SwissProt (Mouse):
Q61490;
SwissProt (Rat):
O35112;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD166 expression in HEK293T (A), HepG2 (B), PMVEC (C), AML12 (D) whole cell lysates. (Predicted band size: 65 kD; Observed band size: 100 kD)
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Immunohistochemical analysis of CD166 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.