Description：Rabbit polyclonal antibody to LeptinImmunogen：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Leptin. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol：LEPAlternative Names：OB; OBS; Leptin; Obese protein; Obesity factor Entrez Gene (Human)： 3952; Entrez Gene (Mouse)： 16846; SwissProt (Human)： P41159; SwissProt (Mouse)： P41160; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Leptin expression in LO2 (A), HCT116 (B), MCF7 (C), A549 (D) whole cell lysates. (Predicted band size: 18 kD; Observed band size: 22 kD)
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  Immunohistochemical analysis of Leptin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.