Description:Rabbit polyclonal antibody to CDK5RAP2Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CDK5RAP2. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol:CDK5RAP2Alternative Names:CEP215; KIAA1633; CDK5 regulatory subunit-associated protein 2; CDK5 activator-binding protein C48; Centrosome-associated protein 215
Entrez Gene (Human):
55755;
Entrez Gene (Mouse):
214444;
SwissProt (Human):
Q96SN8;
SwissProt (Mouse):
Q8K389;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CDK5RAP2 expression in Panc1 (A), Hela (B), mouse heart (C) whole cell lysates. (Predicted band size: 215 kD; Observed band size: 250 kD)
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Immunohistochemical analysis of CDK5RAP2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.