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CPA5927
  • Western blot analysis of TAF1 expression in HepG2 (A), LO2 (B), H1792 (C), AML12 (D), C6 (E) whole cell lysates. (Predicted band size: 212 kD; Observed band size: 250 kD)
  • Immunohistochemical analysis of TAF1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-TAF1 Antibody
Cat No:CPA5927
Source:Rabbit
Reactivity:H, M, R, B, D, P
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Rabbit polyclonal antibody to TAF1
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TAF1. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)
Gene Symbol:TAF1
Alternative Names:BA2R; CCG1; CCGS; TAF2A; Transcription initiation factor TFIID subunit 1; Cell cycle gene 1 protein; TBP-associated factor 250 kDa; p250; Transcription initiation factor TFIID 250 kDa subunit; TAF(II)250; TAFII-250; TAFII250
Entrez Gene (Human): 6872;
Entrez Gene (Mouse): 270627;
SwissProt (Human): P21675;
SwissProt (Mouse): Q80UV9;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of TAF1 expression in HepG2 (A), LO2 (B), H1792 (C), AML12 (D), C6 (E) whole cell lysates. (Predicted band size: 212 kD; Observed band size: 250 kD)
  • Immunohistochemical analysis of TAF1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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