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CPA5867
  • Western blot analysis of PKC mu (Phospho-S205) expression in HEK293T-PMA 10min (A), HEK293T-PMA 30min (B), H9C2 (C), NIH3T3 (D) whole cell lysates. (Predicted band size: 101 kD; Observed band size: 115 kD)
  • Immunohistochemical analysis of PKC mu (Phospho-S205) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-PKC mu (Phospho-S205) Antibody
Cat No:CPA5867
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
420
100 μl
260
30 μl
130
50 μl
170
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Description:Rabbit polyclonal antibody to PKC mu (Phospho-S205)
Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S205 of human PKC mu protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)
Gene Symbol:PRKD1
Alternative Names:PKD; PKD1; PRKCM; Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu
Entrez Gene (Human): 5587;
Entrez Gene (Mouse): 18760;
Entrez Gene (Rat): 85421;
SwissProt (Human): Q15139;
SwissProt (Mouse): Q62101;
SwissProt (Rat): Q9WTQ1;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of PKC mu (Phospho-S205) expression in HEK293T-PMA 10min (A), HEK293T-PMA 30min (B), H9C2 (C), NIH3T3 (D) whole cell lysates. (Predicted band size: 101 kD; Observed band size: 115 kD)
  • Immunohistochemical analysis of PKC mu (Phospho-S205) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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