Description:Rabbit polyclonal antibody to MYOD1 (Phospho-S200)Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S200 of human MYOD1 protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol:MYOD1Alternative Names:BHLHC1; MYF3; MYOD; Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; bHLHc1; Myogenic factor 3; Myf-3
Entrez Gene (Human):
4654;
Entrez Gene (Mouse):
17927;
Entrez Gene (Rat):
337868;
SwissProt (Human):
P15172;
SwissProt (Mouse):
P10085;
SwissProt (Rat):
Q02346;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MYOD1 (Phospho-S200) expression in EC9706 (A), mouse muscle (B) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 45 kD)
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Immunohistochemical analysis of MYOD1 (Phospho-S200) staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.