Description:Rabbit polyclonal antibody to YEATS2Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human YEATS2. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:YEATS2Alternative Names:KIAA1197; YEATS domain-containing protein 2
Entrez Gene (Human):
55689;
Entrez Gene (Mouse):
208146;
SwissProt (Human):
Q9ULM3;
SwissProt (Mouse):
Q3TUF7;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of YEATS2 expression in C6 (A), SP20 (B), A375 (C), Hela (D) whole cell lysates. (Predicted band size: 150 kD; Observed band size: 150 kD)
-
Immunofluorescent analysis of YEATS2 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).