Description:Rabbit polyclonal antibody to PTP1B (Phospho-Y66)Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y66 of human PTP1B protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000)Gene Symbol:PTPN1Alternative Names:PTP1B; Tyrosine-protein phosphatase non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP-1B
Entrez Gene (Human):
5770;
Entrez Gene (Mouse):
19246;
Entrez Gene (Rat):
24697;
SwissProt (Human):
P18031;
SwissProt (Mouse):
P35821;
SwissProt (Rat):
P20417;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PTP1B (Phospho-Y66) expression in MCF7 (A), A549 (B), mouse lung (C) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 50 kD)
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Direct ELISA antibody dose-response curve using Anti-PTP1B (Phospho-Y66) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.