Description:Rabbit polyclonal antibody to Ubiquitin (Phospho-S65)Immunogen:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S65 of human Ubiquitin protein. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000)Gene Symbol:UBA52; RPS27A; UBB; UBCAlternative Names:UBB; Polyubiquitin-B; UBC; Polyubiquitin-C; RPS27A; UBA80; UBCEP1; Ubiquitin-40S ribosomal protein S27a; Ubiquitin carboxyl extension protein 80; UBA52; UBCEP2; Ubiquitin-60S ribosomal protein L40; CEP52; Ubiquitin A-52 residue ribosomal protein fusion product 1
Entrez Gene (Human):
7314;
7316;
6233;
7311;
SwissProt (Human):
P0CG47;
P0CG48;
P62979;
P62987;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Ubiquitin (Phospho-S65) expression in C6 (A), AML12 (B), SGC7901-UV treated (C), SGC7901 (D) whole cell lysates. (Predicted band size: 25; 77; 17; 14 kD; Observed band size: 15; more kD)
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Direct ELISA antibody dose-response curve using Anti-Ubiquitin (Phospho-S65) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.